Cloning of neuronal mtDNA variants in cultured cells by synaptosome fusion with mtDNA-less cells
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چکیده
منابع مشابه
Cloning of neuronal mtDNA variants in cultured cells by synaptosome fusion with mtDNA-less cells.
Synaptosome cybrids were used to confirm the presence of heteroplasmic mtDNA sequence variants in the human brain. Synaptosomes contain one to several mitochondria, and when fused to mtDNA-deficient (rho degrees ) mouse or human cell lines result in viable cybrid cell lines. The brain origin of mouse synaptosome cybrid mtDNAs was confirmed using sequence polymorphisms in the mtDNA COIII, ND3 an...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 2000
ISSN: 1362-4962
DOI: 10.1093/nar/28.10.2164